PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=primer3_config/
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_SALT_DIVALENT=1.5
PRIMER_MAX_SELF_ANY=12
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_MAX_POLY_X=100
PRIMER_MAX_SELF_END=8
PRIMER_PAIR_MAX_COMPL_END=8
P3_FILE_FLAG=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_TASK=pick_pcr_primers
PRIMER_DNTP_CONC=0.0
SEQUENCE_ID=pcr_primers
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
SEQUENCE_TARGET=21,122
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, high hairpin stability 3, ok 0
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=pcr_primers_and_io
PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
SEQUENCE_TEMPLATE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 32, high tm 27, ok 12
PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 11, high tm 38, high hairpin stability 8, ok 17
PRIMER_INTERNAL_EXPLAIN=considered 393, too many Ns 280, low tm 76, high tm 6, long poly-x seq 3, ok 28
PRIMER_PAIR_EXPLAIN=considered 5, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=3.564009
PRIMER_LEFT_0_PENALTY=2.796318
PRIMER_RIGHT_0_PENALTY=0.767691
PRIMER_INTERNAL_0_PENALTY=0.180037
PRIMER_LEFT_0_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_0_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_INTERNAL_0_SEQUENCE=TTAGCGTGCGTGGGGTCCCT
PRIMER_LEFT_0=7,20
PRIMER_RIGHT_0=244,20
PRIMER_INTERNAL_0=142,20
PRIMER_LEFT_0_TM=57.204
PRIMER_RIGHT_0_TM=59.232
PRIMER_INTERNAL_0_TM=60.180
PRIMER_LEFT_0_GC_PERCENT=45.000
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_INTERNAL_0_GC_PERCENT=65.000
PRIMER_INTERNAL_0_SELF_ANY_TH=5.01
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=35.22
PRIMER_INTERNAL_0_HAIRPIN_TH=34.96
PRIMER_LEFT_0_END_STABILITY=4.3000
PRIMER_RIGHT_0_END_STABILITY=4.0000
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=238
PRIMER_PAIR_0_PRODUCT_TM=85.7
PRIMER_PAIR_1_PENALTY=3.818870
PRIMER_LEFT_1_PENALTY=3.051179
PRIMER_RIGHT_1_PENALTY=0.767691
PRIMER_INTERNAL_1_PENALTY=0.180037
PRIMER_LEFT_1_SEQUENCE=GCTGTTTCACACTTACGGAAA
PRIMER_RIGHT_1_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_INTERNAL_1_SEQUENCE=TTAGCGTGCGTGGGGTCCCT
PRIMER_LEFT_1=7,21
PRIMER_RIGHT_1=244,20
PRIMER_INTERNAL_1=142,20
PRIMER_LEFT_1_TM=57.949
PRIMER_RIGHT_1_TM=59.232
PRIMER_INTERNAL_1_TM=60.180
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=5.01
PRIMER_LEFT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=35.22
PRIMER_INTERNAL_1_HAIRPIN_TH=34.96
PRIMER_LEFT_1_END_STABILITY=3.1300
PRIMER_RIGHT_1_END_STABILITY=4.0000
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=238
PRIMER_PAIR_1_PRODUCT_TM=85.7
PRIMER_PAIR_2_PENALTY=3.853878
PRIMER_LEFT_2_PENALTY=2.796318
PRIMER_RIGHT_2_PENALTY=1.057560
PRIMER_INTERNAL_2_PENALTY=0.180037
PRIMER_LEFT_2_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_2_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
PRIMER_INTERNAL_2_SEQUENCE=TTAGCGTGCGTGGGGTCCCT
PRIMER_LEFT_2=7,20
PRIMER_RIGHT_2=247,20
PRIMER_INTERNAL_2=142,20
PRIMER_LEFT_2_TM=57.204
PRIMER_RIGHT_2_TM=58.942
PRIMER_INTERNAL_2_TM=60.180
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=5.01
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=38.11
PRIMER_INTERNAL_2_HAIRPIN_TH=34.96
PRIMER_LEFT_2_END_STABILITY=4.3000
PRIMER_RIGHT_2_END_STABILITY=5.0100
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=241
PRIMER_PAIR_2_PRODUCT_TM=85.8
PRIMER_PAIR_3_PENALTY=3.857112
PRIMER_LEFT_3_PENALTY=2.796318
PRIMER_RIGHT_3_PENALTY=1.060794
PRIMER_INTERNAL_3_PENALTY=0.180037
PRIMER_LEFT_3_SEQUENCE=GCTGTTTCACACTTACGGAA
PRIMER_RIGHT_3_SEQUENCE=CCTGTTGGAGAAAAGCCACT
PRIMER_INTERNAL_3_SEQUENCE=TTAGCGTGCGTGGGGTCCCT
PRIMER_LEFT_3=7,20
PRIMER_RIGHT_3=246,20
PRIMER_INTERNAL_3=142,20
PRIMER_LEFT_3_TM=57.204
PRIMER_RIGHT_3_TM=58.939
PRIMER_INTERNAL_3_TM=60.180
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=5.01
PRIMER_LEFT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=35.22
PRIMER_INTERNAL_3_HAIRPIN_TH=34.96
PRIMER_LEFT_3_END_STABILITY=4.3000
PRIMER_RIGHT_3_END_STABILITY=4.0000
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=240
PRIMER_PAIR_3_PRODUCT_TM=85.9
PRIMER_PAIR_4_PENALTY=4.080246
PRIMER_LEFT_4_PENALTY=3.312555
PRIMER_RIGHT_4_PENALTY=0.767691
PRIMER_INTERNAL_4_PENALTY=0.180037
PRIMER_LEFT_4_SEQUENCE=GCTGTTTCACACTTACGGAAATG
PRIMER_RIGHT_4_SEQUENCE=TGTTGGAGAAAAGCCACTGG
PRIMER_INTERNAL_4_SEQUENCE=TTAGCGTGCGTGGGGTCCCT
PRIMER_LEFT_4=7,23
PRIMER_RIGHT_4=244,20
PRIMER_INTERNAL_4=142,20
PRIMER_LEFT_4_TM=59.687
PRIMER_RIGHT_4_TM=59.232
PRIMER_INTERNAL_4_TM=60.180
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_INTERNAL_4_GC_PERCENT=65.000
PRIMER_INTERNAL_4_SELF_ANY_TH=5.01
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=34.24
PRIMER_RIGHT_4_HAIRPIN_TH=35.22
PRIMER_INTERNAL_4_HAIRPIN_TH=34.96
PRIMER_LEFT_4_END_STABILITY=2.3200
PRIMER_RIGHT_4_END_STABILITY=4.0000
PRIMER_PAIR_4_COMPL_ANY_TH=0.00
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=238
PRIMER_PAIR_4_PRODUCT_TM=85.7
=
SEQUENCE_ID=left_only
PRIMER_TASK=pick_left_only
SEQUENCE_TARGET=100,20
SEQUENCE_TEMPLATE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 34, high tm 809, high hairpin stability 88, ok 129
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.199248
PRIMER_LEFT_0_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
PRIMER_LEFT_0=65,20
PRIMER_LEFT_0_TM=59.801
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.1600
PRIMER_LEFT_1_PENALTY=0.243148
PRIMER_LEFT_1_SEQUENCE=CCTGCAGGTTTAGAAGGGGT
PRIMER_LEFT_1=63,20
PRIMER_LEFT_1_TM=60.243
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY_TH=26.16
PRIMER_LEFT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=0.00
PRIMER_LEFT_1_END_STABILITY=4.9500
PRIMER_LEFT_2_PENALTY=0.382831
PRIMER_LEFT_2_SEQUENCE=ATGCCACGTCCATTTGCTTC
PRIMER_LEFT_2=146,20
PRIMER_LEFT_2_TM=60.383
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=0.00
PRIMER_LEFT_2_END_STABILITY=3.8600
PRIMER_LEFT_3_PENALTY=0.382831
PRIMER_LEFT_3_SEQUENCE=GATGCCACGTCCATTTGCTT
PRIMER_LEFT_3=145,20
PRIMER_LEFT_3_TM=60.383
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY_TH=0.92
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=0.00
PRIMER_LEFT_3_END_STABILITY=3.9100
PRIMER_LEFT_4_PENALTY=0.417433
PRIMER_LEFT_4_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_4=72,20
PRIMER_LEFT_4_TM=59.583
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=0.00
PRIMER_LEFT_4_END_STABILITY=4.6100
=
SEQUENCE_ID=right_only
PRIMER_TASK=PICK_right_only
SEQUENCE_TARGET=90,10
SEQUENCE_TEMPLATE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 279, high tm 102, high hairpin stability 25, ok 149
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_RIGHT_0_PENALTY=0.033659
PRIMER_RIGHT_0_SEQUENCE=CGTGCTTTGCGTTTTGGAAG
PRIMER_RIGHT_0=126,20
PRIMER_RIGHT_0_TM=60.034
PRIMER_RIGHT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_SELF_ANY_TH=0.00
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_RIGHT_0_HAIRPIN_TH=0.00
PRIMER_RIGHT_0_END_STABILITY=3.4600
PRIMER_RIGHT_1_PENALTY=1.023063
PRIMER_RIGHT_1_SEQUENCE=GTGCTTTGCGTTTTGGAAGT
PRIMER_RIGHT_1=125,20
PRIMER_RIGHT_1_TM=58.977
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_SELF_ANY_TH=0.00
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_RIGHT_1_HAIRPIN_TH=0.00
PRIMER_RIGHT_1_END_STABILITY=3.0100
PRIMER_RIGHT_2_PENALTY=1.026309
PRIMER_RIGHT_2_SEQUENCE=GAGCGTGCTTTGCGTTTTG
PRIMER_RIGHT_2=129,19
PRIMER_RIGHT_2_TM=60.026
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_RIGHT_2_SELF_ANY_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_HAIRPIN_TH=37.77
PRIMER_RIGHT_2_END_STABILITY=2.4400
PRIMER_RIGHT_3_PENALTY=1.363507
PRIMER_RIGHT_3_SEQUENCE=GTGCTTTGCGTTTTGGAAGTT
PRIMER_RIGHT_3=125,21
PRIMER_RIGHT_3_TM=59.636
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_SELF_ANY_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_HAIRPIN_TH=0.00
PRIMER_RIGHT_3_END_STABILITY=2.6600
PRIMER_RIGHT_4_PENALTY=1.754055
PRIMER_RIGHT_4_SEQUENCE=TGCTTTGCGTTTTGGAAGTT
PRIMER_RIGHT_4=124,20
PRIMER_RIGHT_4_TM=58.246
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_SELF_ANY_TH=0.00
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_RIGHT_4_HAIRPIN_TH=0.00
PRIMER_RIGHT_4_END_STABILITY=2.6600
=
SEQUENCE_ID=hyb_probe_only
PRIMER_TASK=pick_HYB_probe_ONly
SEQUENCE_INTERNAL_EXCLUDED_REGION=90,20
SEQUENCE_TEMPLATE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_INTERNAL_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 271, high tm 297, high hairpin stability 144, ok 268
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_INTERNAL_0_PENALTY=0.031870
PRIMER_INTERNAL_0_SEQUENCE=GCTCCTGCCTCTCCGGGACA
PRIMER_INTERNAL_0=23,20
PRIMER_INTERNAL_0_TM=59.968
PRIMER_INTERNAL_0_GC_PERCENT=70.000
PRIMER_INTERNAL_0_SELF_ANY_TH=0.00
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_INTERNAL_0_HAIRPIN_TH=30.41
PRIMER_INTERNAL_1_PENALTY=0.111561
PRIMER_INTERNAL_1_SEQUENCE=CCGCGGATGCCACGTCCATT
PRIMER_INTERNAL_1=140,20
PRIMER_INTERNAL_1_TM=60.112
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=20.33
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_INTERNAL_1_HAIRPIN_TH=35.40
PRIMER_INTERNAL_2_PENALTY=0.388807
PRIMER_INTERNAL_2_SEQUENCE=TCCTGCCTCTCCGGGACACC
PRIMER_INTERNAL_2=25,20
PRIMER_INTERNAL_2_TM=59.611
PRIMER_INTERNAL_2_GC_PERCENT=70.000
PRIMER_INTERNAL_2_SELF_ANY_TH=0.00
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_INTERNAL_2_HAIRPIN_TH=30.41
PRIMER_INTERNAL_3_PENALTY=0.602519
PRIMER_INTERNAL_3_SEQUENCE=CCTGCCTCTCCGGGACACCC
PRIMER_INTERNAL_3=26,20
PRIMER_INTERNAL_3_TM=60.603
PRIMER_INTERNAL_3_GC_PERCENT=75.000
PRIMER_INTERNAL_3_SELF_ANY_TH=0.00
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_INTERNAL_3_HAIRPIN_TH=38.95
PRIMER_INTERNAL_4_PENALTY=1.037103
PRIMER_INTERNAL_4_SEQUENCE=CCACAGCCAGCTCTGCCCG
PRIMER_INTERNAL_4=124,19
PRIMER_INTERNAL_4_TM=60.037
PRIMER_INTERNAL_4_GC_PERCENT=73.684
PRIMER_INTERNAL_4_SELF_ANY_TH=0.00
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_INTERNAL_4_HAIRPIN_TH=34.17
=
SEQUENCE_ID=left_only
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.417433
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.583
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.6100
=
SEQUENCE_ID=right_only
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.417433
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.583
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.6100
=
SEQUENCE_ID=hyb_probe_only
PRIMER_INTERNAL_MIN_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=20
PRIMER_TASK=pick_left_only
SEQUENCE_TEMPLATE=TTAGAAGGGGTGTCTGGGTG
PRIMER_WARNING=PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
PRIMER_LEFT_0_PENALTY=0.417433
PRIMER_LEFT_0_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
PRIMER_LEFT_0=0,20
PRIMER_LEFT_0_TM=59.583
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=0.00
PRIMER_LEFT_0_END_STABILITY=4.6100
=
