P3_SETTINGS_FILE_USED=../test/primer_high_tm_load_set.set
P3_FILE_TYPE=settings
P3_FILE_ID=Test with higher Tm
PRIMER_MIN_TM=63
PRIMER_OPT_TM=65
PRIMER_MAX_TM=67
P3_SETTINGS_FILE_END=
SEQUENCE_ID=Load qPCR settings
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=5
PRIMER_TASK=pick_detection_primers
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.107238
PRIMER_LEFT_0_PENALTY=0.030618
PRIMER_RIGHT_0_PENALTY=0.076620
PRIMER_LEFT_0_SEQUENCE=accgtgcaccggaatcagtt
PRIMER_RIGHT_0_SEQUENCE=cggaaaaccagcctccaatg
PRIMER_LEFT_0=1030,20
PRIMER_RIGHT_0=1205,20
PRIMER_LEFT_0_TM=65.031
PRIMER_RIGHT_0_TM=64.923
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=6.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=3.00
PRIMER_RIGHT_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=6.7000
PRIMER_RIGHT_0_END_STABILITY=7.2000
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=2.00
PRIMER_PAIR_0_PRODUCT_SIZE=176
PRIMER_PAIR_0_PRODUCT_TM=74.2
PRIMER_PAIR_1_PENALTY=0.168388
PRIMER_LEFT_1_PENALTY=0.051236
PRIMER_RIGHT_1_PENALTY=0.117152
PRIMER_LEFT_1_SEQUENCE=ggttttccgatgcagaaccg
PRIMER_RIGHT_1_SEQUENCE=aaagctcaccggaactcccc
PRIMER_LEFT_1=1197,20
PRIMER_RIGHT_1=1364,20
PRIMER_LEFT_1_TM=64.949
PRIMER_RIGHT_1_TM=64.883
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=168
PRIMER_PAIR_1_PRODUCT_TM=78.8
PRIMER_PAIR_2_PENALTY=0.191614
PRIMER_LEFT_2_PENALTY=0.124657
PRIMER_RIGHT_2_PENALTY=0.066957
PRIMER_LEFT_2_SEQUENCE=cacaaatggtccccacgaca
PRIMER_RIGHT_2_SEQUENCE=gcctgccggtttcttgtacg
PRIMER_LEFT_2=581,20
PRIMER_RIGHT_2=876,20
PRIMER_LEFT_2_TM=65.125
PRIMER_RIGHT_2_TM=65.067
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PAIR_2_PRODUCT_SIZE=296
PRIMER_PAIR_2_PRODUCT_TM=79.5
PRIMER_PAIR_3_PENALTY=0.193772
PRIMER_LEFT_3_PENALTY=0.076620
PRIMER_RIGHT_3_PENALTY=0.117152
PRIMER_LEFT_3_SEQUENCE=cattggaggctggttttccg
PRIMER_RIGHT_3_SEQUENCE=aaagctcaccggaactcccc
PRIMER_LEFT_3=1186,20
PRIMER_RIGHT_3=1364,20
PRIMER_LEFT_3_TM=64.923
PRIMER_RIGHT_3_TM=64.883
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PAIR_3_PRODUCT_SIZE=179
PRIMER_PAIR_3_PRODUCT_TM=79.0
PRIMER_PAIR_4_PENALTY=0.197675
PRIMER_LEFT_4_PENALTY=0.124657
PRIMER_RIGHT_4_PENALTY=0.073018
PRIMER_LEFT_4_SEQUENCE=cacaaatggtccccacgaca
PRIMER_RIGHT_4_SEQUENCE=cctgccggtttcttgtacgg
PRIMER_LEFT_4=581,20
PRIMER_RIGHT_4=875,20
PRIMER_LEFT_4_TM=65.125
PRIMER_RIGHT_4_TM=64.927
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=295
PRIMER_PAIR_4_PRODUCT_TM=79.4
=
